The exome represents less than two percent of the human genome but contains about 85% of known disease-causing variants. Whole-exome sequencing refers to sequencing all of the protein-coding genes in a genome. The adequate sequencing platform for your project will depend on sequencing depth (sequencing throughput) and read length required for your project. PacBio sequencers can also be used for amplicons sequencing and whole genome sequencing either in combination with Illumina sequencing or by itself. We recommend contacting us before initiating a project with us.Īll of the services listed below are compatible with current Illumina sequencing platforms. Most next generation sequencing projects require special consideration. Contact us with questions regarding experimental design or DNA service options. Before submitting samples, please review our sample submission requirements.Ĭhoosing the correct DNA sequencing service for your samples depends on the organism, total DNA available for input, and project goals. DNA submitted for PacBio sequencing will further be run on TapeStation or Fragment Analyzer to check integrity of the DNA. Upon delivery to our facility, all DNA samples are evaluated for concentration by Qubit®. If you do not find the DNA sequencing service you need for your project in the list below, please contact us. The Sequencing and Genomic Technology shared resource provides a large array of DNA sequencing services, such as Whole Genome Sequencing, Whole Exome Sequencing or Targeted sequencing.
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